TAN #121: Oh hai! kTANxbai. (Page 21)

I don't know how you feel about ... "AAAAAAAAAAAAARGH!"

You have a fantastic fiance and getting married.  That's good


Edited by Kido 2010-10-05 11:43 AM

{{{MELON PRESS}}} that should hit you just in the right place

Indeed, that is very good indeed.

<3 u 2

Hehe, nice one, Cord. 

I think I'm going to bribe myself to get work done: get the experiment set up, get some good coffee.  Then I will be warm and more awake.

Have bad coffee so that you CAN get the expt set up, with the understanding you will then reward yourself with GOOD coffee.

I've had most of it.  I have to be careful - if I have too much, my hands get shaky and that's not good for dealing with the tiny (0.2 mL) tubes.

That's so funny. I was just thinking of shaky hands and pipetting into gel wells this morning. Ruined many a lovely gel. Usually, of course, on the last well or two.

I've done that many times.  Usually I poke through them or tear the sides.  But I almost always have more wells than I need.

The worst is SDS-PAGE gels  that are vertical and have tiny openings for their well.  Makes agarose gels a piece of cake.

Ah yes I've spent many an afternoon bent over a bench loading up a denaturing PAGE gel and burning my hands pulling those little bitty tubes out of the heating block, fun times. 

eta: and mmmmm the smell of Tmed. 


Edited by trinnas 2010-10-05 12:21 PM

Ah...good times.

On that note, I'm off to do just that.

Aaaah, the memories. Sometimes I really miss this stuff. But I really can't spend a significant portion of my life in a lab or office--bad things happen, and not just a ruined gel or two.

HYPERPUPPY HYPERPUPPY HYPERPUPPY!

Although ... I'm beginning to think that everything people say about dogs is actually true of cats, too. Mine are stinky and slobbery and hyper and try to hump (well, nurse) my leg and they're very vocal and like to sniff and lick each other's butts.

I could never get coomassie to stain properly. Always ended up running duplicate gels and silver staining the second one after the coomassie didn't develop properly. Load the crap out of it and still the bands were weak.

Westerns: Whole nuther set of problems there. GAWD!

Edited by pitt83 2010-10-05 12:23 PM

Hypnomelons!!! {{{MELON PRESS}}}

If someone shakes while doing manual tasks, what could that be?

It sort of looked like they were straining, as if they had to apply a lot of force and shaking, but it was pretty easy...

MS?  Drinking problem?  There is no noticable shake day to day at the desk...

Yanti Melon Goddess and Siren Sylph how are you this...... um..... morning?

SIREN SYLPH!!!!!! Now there's a smile.

I said earlier, I haz a sad, but it's a good sad, mostly. Just enjoying time with my mom.

Feeling a bit better. Now just very tired/fatigued/headache/bodyache, but I don't actually feel *sick* anymore.

Bumming right now.
Found out a few minutes ago that, Jeff Littman, a local bike advocate, President of the Wisconsin Cycling Association, and owner of a popular bike shop (Attitudes) out of the Pewaukee/Milwaukee, WI, area, may have passed away from his injuries that he sustained from the car that hit him a couple of days ago -
http://www.beginnertriathlete.com/discussion/forums/thread-view.asp?tid=229077&posts=70#M3133867

Why does shat like this always happen?  Makes me sick.

Enjoying the time now is the best way to honor the time you had IMHO

I have to admit I'm feeilng pretty run down myself and a little sad too.  I am glad you are feeling better. 

Jim, it could probably be a whole lot of things.  Could be any of the above, too much coffee, nerves, and I think some people are just shakey people.  Why do you ask? 

Oh man...that's really sad news. Jeff was a good guy.  Really friendly and knowledgeable. 

Soprry to hear about it, Phil.  That's balls.  Not cool at all. 

I'm just a shaky person.  Add to that too much caffeine and I am really bad. 

That is sad news sorry to hear it Phil.

Jim: Loading a gel is pretty tricky. You're trying to put a 2mm tip in a 5mm slot while holding the pipette about 9inches above where you're putting the tip. Plus looking through a layer of plastic and a tank full of water. Plus it's made out of gel, so go too deep or left or right too far and you've f'd it up. And the samples are warm and tips clog. PIA in lab work for sure.